GRP4 Lasius Niger

Sample information
Picture	Photos by: Katherine L., Parishi J., Ritu G., Emma L.
Location
Collection date	05/14/2024
Captive / Cultivated?	Wild-caught
Group	California Academy of Mathematics and Science
Observations	Found near the soil of the garden, dark brown/black color, around a few other ants
Putative identification	Arthropoda Hexapoda Insecta
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	Fast Blast
Gel images
Protocol notes	Ran the gel for 60 minutes at 105 volts.
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	Our confidence level was determined by the success in our DNA Ladder and controls. The Arthropod CO1 and Wolbachia-specific 16S rRNA genes were present in both the (+) Arthropod control and (+) DNA control in our Arthropod PCR samples. Additionally, the (-) Arthropod control contained only the CO1 band and no Wolbachia band in our Arthropod PCR side, and there were no bands or smudges in our (-) water controls. This indicates that the extraction and PCR processes of extracting the genes were successful.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Insecta was found to be negative for Wolbachia.

The Wolbachia Project Database