Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/14/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | California Academy of Mathematics and Science |
| Observations | Squirming behind laundry basket with lots of dust. |
| Putative identification | Arthropoda Insecta |
Methods |
|
| Extraction kit | tweezers |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | UView |
| Gel images |
|
| Protocol notes | Gel was ran for close to an hour. Visualized immediately after using UV light. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | No bands were present for negative control, while bands were present for positive control. Meaning our data from the gel should be accurate. However, the bands are faint. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Insecta was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid