GRP10 Lepisma saccharina

May 25, 2024

Sample information
Picture
Photos by: Mikaela G.,
Location
Collection date 05/14/2024
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

Squirming behind laundry basket with lots of dust.
Putative identification Arthropoda Hexapoda Insecta

Methods
Extraction kit tweezers
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain UView
Gel images
Protocol notes

Gel was ran for close to an hour. Visualized immediately after using UV light.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

No bands were present for negative control, while bands were present for positive control. Meaning our data from the gel should be accurate. However, the bands are faint.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Insecta was found to be negative for Wolbachia.
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