GRP10_Drosophila melanogaster (Fruit Fly)

May 25, 2024

Sample information
Picture
Photos by: Mikaela G
Location
Collection date 05/14/2024
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

Arthropod was caught on a raspberry, left as a trap, and put into freezer to slow it down.
Putative identification Arthropoda Hexapoda Insecta Diptera

Methods
Extraction kit tweezers
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain UView
Gel images
Protocol notes

Ran for about an hour at 125V and visualized immediately after (avoid UV dissipating) using UV light.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Many people were in the lab (very crowded) pipets and tools might have been contaminated. Although controls are visible, we aren’t 100% certain what the controls are meant to look like. And ladder is very faint so estimating base pairs could be incorrect.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be negative for Wolbachia.
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