Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/14/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | California Academy of Mathematics and Science |
| Observations | Arthropod was caught on a raspberry, left as a trap, and put into freezer to slow it down. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera |
Methods |
|
| Extraction kit | tweezers |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | UView |
| Gel images |
|
| Protocol notes | Ran for about an hour at 125V and visualized immediately after (avoid UV dissipating) using UV light. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | Many people were in the lab (very crowded) pipets and tools might have been contaminated. Although controls are visible, we aren’t 100% certain what the controls are meant to look like. And ladder is very faint so estimating base pairs could be incorrect. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Diptera was found to be negative for Wolbachia. |
Water Boatman