Red-Banded Fungus Beetle

November 5, 2020

Sample information
Picture
Photos by: Mavis W.
Location
Collection date 09/24/2020
Captive / Cultivated? Wild-caught
Group Hillsboro High School
Observations

The beetle was found with many of the same species on a stump. It was extracted from the stump, which was damp and dark. It was 5:30 pm and around 70 degrees Fahrenheit.
Putative identification Arthropoda Hexapoda Insecta Coleoptera Erotylidae Megalodacne

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

Not confident because the extraction process may have been affected by the durability of the insect’s exoskeleton.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Megalodacne was found to be negative for Wolbachia.
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