Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/24/2020 |
| Captive / Cultivated? | Wild-caught |
| Group | Hillsboro High School |
| Observations | The beetle was found with many of the same species on a stump. It was extracted from the stump, which was damp and dark. It was 5:30 pm and around 70 degrees Fahrenheit. |
| Putative identification | Arthropoda Insecta Coleoptera Erotylidae Megalodacne |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | Not confident because the extraction process may have been affected by the durability of the insect’s exoskeleton. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Megalodacne was found to be negative for Wolbachia. |
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid
Blattella germanica