Red-Banded Fungus Beetle

Sample information

Picture
Photos by: Mavis W.
Location
Collection date 09/24/2020
Captive / Cultivated? Wild-caught
Group Hillsboro High School
Observations

The beetle was found with many of the same species on a stump. It was extracted from the stump, which was damp and dark. It was 5:30 pm and around 70 degrees Fahrenheit.

Putative identification Hexapoda Insecta Coleoptera Erotylidae Megalodacne

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level Low
Explanation of confidence level

Not confident because the extraction process may have been affected by the durability of the insect’s exoskeleton.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Megalodacne was found to be negative for Wolbachia.
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