Common woodlouse

Sample information

Picture
Photos by: Cal N, Colleen N.
Location
Collection date
Captive / Cultivated? Captive / Cultivated
Group Hillsboro High School
Observations

This arthropod was found underneath a rock in my front yard, along with several other insects of varying species. This was done in the afternoon, fairly warm out too but I do not have an exact measurement of the temperature. It was the only roly poly I managed to find. It was curled up, probably in an attempt to hide or protect itself from me, however, it had the opposite effect as I was able to grab it and submerge it in ethanol easier than if it were moving around.

Putative identification Crustacea Malacostraca Isopoda Armadillidae

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Gel Electrophoresis Review – Cal Neel

My results are labeled under number 27 and 26. 27 was a common woodlouse and 26 was a carpenter ant. My pill bug tested positive for Wolbachia, however, my ant definitely did not.

Results

Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I am very confident in these results since the DNA ladders included alongside the arthropod DNA matched as they should, so the controls worked perfectly. One of my bands (number 27) is at the length of typical Wolbachia positive bands (500 to 430 base pairs long), and my band is just under 500 base pairs.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Armadillidae was found to be postive for Wolbachia.
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