Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 06/03/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Pingry School |
| Observations | Slightly-red tint on abdomen, very long antennae, ant-like motion caught in a tree in a forest outside of the Pingry school. Camponotus nearcticus- neartic carpenter ant
|
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | missing arthropod DNA due to unsuccessful sequencing |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | no Wolbachia data- there are no DNA fragments in the corresponding lane. In Wolbachia sequencing, there were no clear base pairs after adding the Wolbachia primers because Wolbachia is not present in the DNA of the Agonum punctiforme. |
| Wolbachia 16S sequence | Download AB1
|
| Arthropod COI sequence |
|
| Summary | The Formicidae was found to be negative for Wolbachia. |
Thrip – Phlaeothripidae Leptothrips sp. 21
MACALF24-SB Dermaptera
Thrip – Phlaeothripidae Leptothrips sp. 19