Camponotus chromaiodes

Sample information

Photos by: Aanvi T
Collection date 06/03/2024
Captive / Cultivated? Wild-caught
Group Pingry School

The Camponotus chromaiodes was found on a dry, dusty, sunlit part of the path into the forest. The ground it was found on was littered with cracks and holes where many similar ants of its species were ducking in and out. The Camponotus chromaiodes was extremely fast and seemed to be a bit smaller for its kind, indicating that it wasn’t fully developed. Upon further observation under a microscope, we could see that the Camponotus chromaiodes had a smaller abdomen and very large antennae with a distinctly rusty color along its mesosoma and legs.

Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Camponotus Camponotus chromaiodes


Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

In order to begin our DNA extraction, we had to dissect the abdomen of the Camponotus chromaiodes and wash it under running water for two minutes to remove the ethanol present. We went through the steps of cell lysis and precipitation, DNA purification, and DNA elution. After that, we checked our concentrations with a nanodrop and finished PCR while setting up our gel. After about 15-20 minutes, we checked on our gel and visualized our results (see gel above). My arthropod was in Lane 3. The top lane contained our Arthropod samples, and the bottom lane contained our Wolbachia samples, which helped us determine that our bug contained Wolbachia (see gel above).


Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The Wolbachia band on my gel was extremely bright, and the high-quality values on my sequence confirmed that the Camponotus chromaiodes had Wolbachia. I am confident that the sequence data was very accurate, as the peaks on my sequence were very distinct.

Wolbachia 16S sequence Download FASTA    Download AB1
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Camponotus chromaiodes was found to be postive for Wolbachia.
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