Scolopocryptops sexspinosus (centipede)

June 7, 2024

Sample information
Picture
Photos by: Daniel L.
Location
Collection date 06/03/2024
Captive / Cultivated? Wild-caught
Group Pingry School
Observations

This arthropod was found near a fallen tree inside the forest at the Pingry Campus in Basking Ridge, New Jersey.
Putative identification Arthropoda Myriapoda Chilopoda Scolopendromorpha Scolopocryptopidae Scolopocryptops Scolopocryptops sexspinosus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

The SEEK app was used to identify the insect’s species. The insect was crushed and used in its entirety. After identifying it, its DNA was extracted, eluted, run through PCR, and run through the gel.

The top section of the gel is the control for the COI gene in arthropods, and the bottom section of the gel tests for Wolbachia amplification.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

The controls seemed to work correctly.  The section at the top was a bit faint, but the results could still be read. The section at the bottom is robust, which gives me more confidence that the millipede was infected with Wolbachia.
Wolbachia 16S sequence Download FASTA    Download AB1
GTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
TTGAGCATCAATAGCAGGAACTGCTCTTAGCCTAATTATCCGTTTAGAATTGAGTCAACCGGGAACCCTAATTGGTGATGATCAAACTTACAATACTATTGTAACTGCTCACGCATTTGTAATAATTTTCTTTATAGTAATACCAATTATAATTGGAGGATTCGGAAATTGATTAACCCCTTTAATACTAGGAGCTCCTGATATAGCTTTCCCTCGACTTAATAATATAAGTTTTTGATTACTTCCTCCTTCATTAATACTTTTAATAGGATCAGCTATAGTTGAAAGAGGAGCCGGAACAGGATGAACAGTATACCCTCCTCTAGCAGCTAATCTTGCACACTCAGGACCTTCAGTAGATATAACAATTTTTTCATTACATCTTGCTGGAGTTTCTTCTATTTTAGGAGCTATTAATTTTATTACAACTATTATTAATATACGAACAAGAGGAATAGTAATAGAACGAGTTCCCCTTTTTGTCTGGGCAGTATTAATCACCACCATCCTTCTTTTATTATCTCTCCCTGTATTAGCAGGAGCAATTACNATATTACTTACAGATCGAAATTTTAATACTAGATTTTTTGATCCAGCAGGAGGAGGGGATCCTATTTTATACCAACATTTATTTTGATTTTTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Scolopocryptops sexspinosus was found to be postive for Wolbachia.
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