Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 06/03/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Pingry School |
| Observations | This arthropod was found near a fallen tree inside the forest at the Pingry Campus in Basking Ridge, New Jersey. |
| Putative identification | Arthropoda Myriapoda Chilopoda Scolopendromorpha Scolopocryptopidae Scolopocryptops Scolopocryptops sexspinosus |
Methods |
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| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
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| Protocol notes | The SEEK app was used to identify the insect’s species. The insect was crushed and used in its entirety. After identifying it, its DNA was extracted, eluted, run through PCR, and run through the gel. The top section of the gel is the control for the COI gene in arthropods, and the bottom section of the gel tests for Wolbachia amplification. |
Results |
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| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | The controls seemed to work correctly. The section at the top was a bit faint, but the results could still be read. The section at the bottom is robust, which gives me more confidence that the millipede was infected with Wolbachia. |
| Wolbachia 16S sequence | Download FASTA
Download AB1
GTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTT
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence | Download FASTA
Download AB1
TTGAGCATCAATAGCAGGAACTGCTCTTAGCCTAATTATCCGTTTAGAATTGAGTCAACCGGGAACCCTAATTGGTGATGATCAAACTTACAATACTATTGTAACTGCTCACGCATTTGTAATAATTTTCTTTATAGTAATACCAATTATAATTGGAGGATTCGGAAATTGATTAACCCCTTTAATACTAGGAGCTCCTGATATAGCTTTCCCTCGACTTAATAATATAAGTTTTTGATTACTTCCTCCTTCATTAATACTTTTAATAGGATCAGCTATAGTTGAAAGAGGAGCCGGAACAGGATGAACAGTATACCCTCCTCTAGCAGCTAATCTTGCACACTCAGGACCTTCAGTAGATATAACAATTTTTTCATTACATCTTGCTGGAGTTTCTTCTATTTTAGGAGCTATTAATTTTATTACAACTATTATTAATATACGAACAAGAGGAATAGTAATAGAACGAGTTCCCCTTTTTGTCTGGGCAGTATTAATCACCACCATCCTTCTTTTATTATCTCTCCCTGTATTAGCAGGAGCAATTACNATATTACTTACAGATCGAAATTTTAATACTAGATTTTTTGATCCAGCAGGAGGAGGGGATCCTATTTTATACCAACATTTATTTTGATTTTTG
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Scolopocryptops sexspinosus was found to be postive for Wolbachia. |
Water Boatman