Sample 1 – Mosquito

November 24, 2020

Sample information
Picture
Photos by: Kaitlynn C.
Location No map location data available.
Collection date 10/04/2020
Captive / Cultivated? Wild-caught
Group Toms River High School East
Observations

When I caught the mosquito it was more difficult than expected. Every time I went to go grab one it flew away. When I caught it I looked at it and the legs were very long and the body was very small.
Putative identification Arthropoda Insecta Diptera Culicidae Culex

Methods
Extraction kit
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

I have no idea if this mosquito has Wolbachia I kind of just guessed that it did.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Culex was found to be postive for Wolbachia.
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