Flocculent Tailed Millipede

Sample information

Picture
Photos by: Michelle G.
Location
Collection date 11/12/2024
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

The arthropod is long and has many, many legs (over a hundred pairs) with two pairs per segment. It is of a dark brown color with a segmented exoskeleton. The underbelly is a cream color, distinguishing it from many other millipede species. It has two visible eyes and antennae that are bent. The body is segmented, but it does not have a thorax or an abdomen; therefore, it is not an insect. It is about an inch and a half long.

Putative identification Arthropoda Myriapoda Diplopoda Julida Parajulidae Ptyoiulus Ptyoiulus impressus

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

No visible bands appeared in the prospective millipede wells for both the Wolbachia and the arthropod gel electrophoresis. Since it is not evident that there is enough genetic material to analyze, the results are inconclusive, so this sample was not sent in for sequencing.

Results

Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

Results are inconclusive since PCR and gel electrophoresis were unsuccessful. Sequencing was not run, so identification cannot be determined. Therefore, I am not confident about what the species is.

Wolbachia 16S sequence
Arthropod COI sequence
Summary
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