|Captive / Cultivated?||Wild-caught|
|Group||Venture High School|
The Stone Dwelling centipede has a more circular head that has a number of simple eyes. These are darkly pigmented and when they are numerous, they form a dark patch on each side of their head. Their antennae are quite long, usually at least one-third of the length of the body. Predator to small insects and other smaller arthropods, prey to shrews, toads, badgers, and birds.
|Putative identification||Chilopoda Lithobiomorpha|
|Extraction kit||DNeasy (Qiagen)|
|DNA extraction location||Whole arthropod|
|Single or Duplex PCR|
|Gel electrophoresis system||MiniPCR|
We followed the recommended conditions for Wolbachia primers only. We used the standard reagents from MiniPCR.
|Explanation of confidence level||
My control wasn’t that bright but it was easy enough to tell.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Lithobiomorpha was found to be negative for Wolbachia.|