stone centipede

Sample information

Picture
Photos by: Malachi S, T Edwards
Location
Collection date 03/23/2021
Captive / Cultivated? Wild-caught
Group Venture High School
Observations

The Stone Dwelling centipede has a more circular head that has a number of simple eyes. These are darkly pigmented and when they are numerous, they form a dark patch on each side of their head. Their antennae are quite long, usually at least one-third of the length of the body. Predator to small insects and other smaller arthropods, prey to shrews, toads, badgers, and birds.

Putative identification Chilopoda Lithobiomorpha

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

We followed the recommended conditions for Wolbachia primers only. We used the standard reagents from MiniPCR.

Results

Wolbachia presence No
Confidence level Medium
Explanation of confidence level

My control wasn’t that bright but it was easy enough to tell.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lithobiomorpha was found to be negative for Wolbachia.
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