Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/11/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations |
|
| Putative identification | Arthropoda Chelicerata Arachnida Araneae Lycosidae Pardosa Pardosa lugubris |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Fast Blast |
| Gel images |
|
| Protocol notes | Negative result on line VF1 |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | All the controls worked as expected. There have not been any problems with the protocol and the bands of the controls appeared where they should. Therefore we are confident that the antropod is not infected with Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Pardosa lugubris was found to be negative for Wolbachia. |
Water Boatman