Pardosa lugubris – VF1

April 8, 2021

Sample information
Picture
Photos by: Samira S., Vivien F.
Location
Collection date 03/11/2021
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

Putative identification Arthropoda Chelicerata Arachnida Araneae Lycosidae Pardosa Pardosa lugubris

Methods
Extraction kit Edwards Buffer
DNA extraction location Partial abdomen
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

Negative result on line VF1
Wolbachia positive on line WB+

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All the controls worked as expected. There have not been any problems with the protocol and the bands of the controls appeared where they should. Therefore we are confident that the antropod is not infected with Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Pardosa lugubris was found to be negative for Wolbachia.
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