|Captive / Cultivated?||Wild-caught|
|Group||Hampton Roads Academy|
6 legs, segmented, wings, antenas, furry.
|Putative identification||Hexapoda Insecta Coleoptera|
|Extraction kit||Edwards Buffer|
|DNA extraction location||Abdomen|
|Single or Duplex PCR|
|Gel electrophoresis system||MiniOne|
The pellet was very large and dark.
|Explanation of confidence level||
My line was very faint and this is my first time extracting DNA.
Arthropod control -A didn’t seem to work. -A and +A control in Wolbachia gels didn’t seem to work.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Coleoptera was found to be postive for Wolbachia.|