Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/02/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | 6 legs, segmented, wings, antenas, furry. |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | The pellet was very large and dark. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | My line was very faint and this is my first time extracting DNA. Arthropod control -A didn’t seem to work. -A and +A control in Wolbachia gels didn’t seem to work. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Coleoptera was found to be postive for Wolbachia. |
Water Boatman