Roly-Poly

May 4, 2021

Sample information
Picture
Photos by: Ellie G.
Location
Collection date 03/30/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

It was rolling around in the dirt and rolled up when I touched it into a ball.

Small , 10 legs, brown and black, has a hard shell with scales
Antennas, black eyes
Putative identification Arthropoda Crustacea Malacostraca Isopoda

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Pellet was visible.

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

The band was faint.

First time doing the experiment, not sure about +A band.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Isopoda was found to be postive for Wolbachia.
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