Roly-poly

May 4, 2021

Sample information
Picture
Location
Collection date 04/01/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

My roly-poly had 14 legs, dark bumpy eyes, grooves on its back, antennas, and spots on its back.
Putative identification Arthropoda Crustacea Malacostraca Isopoda

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

During Cell Lysis, when we placed our DNA in the very hot water, my cap popped open, but it didn’t look like anything spilled. The pellet was still inside, so I kept going. Another error I made was that I put double the amount of isopropanol.

My pellet was a small speck that looked murky and dark.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

First time doing this experiment, not sure about the controls and I’m also unsure if I had an A+ band.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Isopoda was found to be postive for Wolbachia.
Report Inappropriate Post