Roly poly

May 4, 2021

Sample information
Picture
Location
Collection date 03/31/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

My roly poly was brown and striped. It had 14 legs, 2 antennas, and dark, beady eyes.
Putative identification Arthropoda Crustacea Malacostraca Isopoda

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

During my experiment, I had to grind my sample, but it was difficult. My roly poly kept getting stuck on the bottom. I was able to move it to the side and grind it, but I still may not have ground it enough.

My pellet was small. It was only a few grey specks.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

My confidence is low because we ran out of the GelGreen, so we used regular gel. This gel did not allow the bands to be clear. First time doing the experiment, not sure about +A band.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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