Moth

May 5, 2021

Sample information
Picture
Photos by: Christian C.
Location
Collection date 03/30/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

The moth was gray and small. It stayed mostly in place on the surface they were on. It had 2 sets of wings with a long thin golden stripe running along the wing. It had small antennae. It had large eyes and a fuzzy head.
Putative identification Arthropoda Hexapoda Insecta Lepidoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The pellet was large and clearly visible as well as ovular in shape.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

The arthropod control didn’t seem to work, and this is my first time extracting DNA.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lepidoptera was found to be negative for Wolbachia.
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