Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/30/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | The moth was gray and small. It stayed mostly in place on the surface they were on. It had 2 sets of wings with a long thin golden stripe running along the wing. It had small antennae. It had large eyes and a fuzzy head. |
| Putative identification | Arthropoda Hexapoda Insecta Lepidoptera |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | The pellet was large and clearly visible as well as ovular in shape. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | The arthropod control didn’t seem to work, and this is my first time extracting DNA. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Lepidoptera was found to be negative for Wolbachia. |
Water Boatman