Roly-Poly

May 6, 2021

Sample information
Picture
Location
Collection date 04/01/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

brown and speckled shell, white underbelly
Putative identification Arthropoda Crustacea Malacostraca Isopoda

Methods
Extraction kit Edwards Buffer
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

My pellet was brown and chunky, this could show that I didn’t grind my pellet enough. My supernatant was light clear brown.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

My line was strong for wolbachia, but my role-poly’s DNA wasn’t tested for being an arthropod.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Isopoda was found to be postive for Wolbachia.
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