|Captive / Cultivated?||Wild-caught|
|Group||Hampton Roads Academy|
brown and speckled shell, white underbelly
|Putative identification||Crustacea Malacostraca Isopoda|
|Extraction kit||Edwards Buffer|
|DNA extraction location|
|Single or Duplex PCR|
|Gel electrophoresis system||MiniPCR|
My pellet was brown and chunky, this could show that I didn’t grind my pellet enough. My supernatant was light clear brown.
|Explanation of confidence level||
My line was strong for wolbachia, but my role-poly’s DNA wasn’t tested for being an arthropod.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Isopoda was found to be postive for Wolbachia.|