Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/01/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | brown and speckled shell, white underbelly |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | My pellet was brown and chunky, this could show that I didn’t grind my pellet enough. My supernatant was light clear brown. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | My line was strong for wolbachia, but my role-poly’s DNA wasn’t tested for being an arthropod. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Isopoda was found to be postive for Wolbachia. |
Water Boatman