Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/02/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | It looked like a normal roly-poly. It was still alive when I collected it. It had a hard shell and 14 legs; and it was a dark brown color. |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | The pellet was very small and hard to see. It looked clear though. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | DNA ladder in arthropod gel was not loaded properly. Positive and negative controls did not work. Wolbachia gel control did not work. Maybe problems with DNA extraction. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Isopoda was found to be negative for Wolbachia. |
Water Boatman