|Captive / Cultivated?||Wild-caught|
|Group||Hampton Roads Academy|
It looked like a normal roly-poly. It was still alive when I collected it. It had a hard shell and 14 legs; and it was a dark brown color.
|Putative identification||Crustacea Malacostraca Isopoda|
|Extraction kit||Edwards Buffer|
|DNA extraction location||Abdomen|
|Single or Duplex PCR|
|Gel electrophoresis system||MiniOne|
The pellet was very small and hard to see. It looked clear though.
|Explanation of confidence level||
DNA ladder in arthropod gel was not loaded properly. Positive and negative controls did not work. Wolbachia gel control did not work. Maybe problems with DNA extraction.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Isopoda was found to be negative for Wolbachia.|