Sample information

Photos by: Zoe W.
Collection date 03/30/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy

Yellow lines/stripes on the abdomen

very big abdomen

6 legs

2 antennas

Putative identification Arthropoda Hexapoda Insecta Hymenoptera


Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

DNA extraction didn’t squeeze bug enough

pipetted tiny tubes wrong

not enough supernatant

the pellet was very small but visible



Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

They may have been a switch between the Wolbachia DNA and the negative arthropod control in the Wolbachia band the negative arthropod control should not have a band.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be postive for Wolbachia.
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