Ant

May 4, 2021

Sample information
Picture
Photos by: Tyler S
Location
Collection date 03/30/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

It is a very small ant with dark body and has 6 light brown limbs
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Arthropod DNA latter is faint
2 has arthropod band
No band for beetle
4 has band
11,12,13 have bands
Band on 5
DNA walbechia dna later is much better
Positive and negative walbechia no band
Not sure if positive control gave a band
No walbechia have bands, it is messed up

 

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

Dna latter is faint

positive or negative controls did not work

walbechia gel positive control did not work, may be problems with dna extraction
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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