Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/20/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | The Summit Country Day School |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera Cicadidae Magicicada Magicicada septendecim |
Methods |
|
| Extraction kit | Thermoscientific GeneJet Genomic Purification |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | All controls worked; presence of Wolbachia PCR product in both duplex and single PCR reactions; sequence results of Wolbachia PCR product identifies as wolbachia wMel (supergroup A) and CO1 PCR product identifies as Magicicada decim. |
| Wolbachia 16S sequence | Download FASTA
|
| Arthropod COI sequence | Download AB1
|
| Summary | The Magicicada septendecim was found to be postive for Wolbachia. |
Millipede
Cylindroiulus caeruleocinctus
Biting Midge
Eastern Red Centipede
Specimen_3