Cicada 29

Sample information

Photos by: Dr. Replogle
Collection date 05/20/2021
Captive / Cultivated? Wild-caught
Group The Summit Country Day School
Putative identification Arthropoda Hexapoda Insecta Hemiptera Cicadidae Magicicada Magicicada septendecim


Extraction kit Thermoscientific GeneJet Genomic Purification
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes


Wolbachia presence Yes
Confidence level High
Explanation of confidence level

All controls worked; presence of Wolbachia PCR product in both duplex and single PCR reactions; sequence results of Wolbachia PCR product identifies as wolbachia wMel (supergroup A) and CO1 PCR product identifies as Magicicada decim.

Wolbachia 16S sequence Download FASTA   
Arthropod COI sequence Download AB1
Summary The Magicicada septendecim was found to be postive for Wolbachia.
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