Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 07/07/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Norwich Free Academy |
| Observations | Near a stream. Caught on grass. Long |
| Putative identification | Arthropoda Insecta Dermaptera Anisolabididae Euborellia Euborellia annulipes |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Fast Blast |
| Gel images |
|
| Protocol notes | I mashed the entire bug |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | I did not see any stain lines. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Euborellia annulipes was found to be negative for Wolbachia. |


American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid
Blattella germanica