Ring legged Earworm

July 16, 2021

Sample information
Picture
Photos by: JunoY
Location
Collection date 07/07/2021
Captive / Cultivated? Wild-caught
Group Norwich Free Academy
Observations

Near a stream. Caught on grass. Long
Putative identification Arthropoda Hexapoda Insecta Dermaptera Anisolabididae Euborellia Euborellia annulipes

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

I mashed the entire bug

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I did not see any stain lines.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Euborellia annulipes was found to be negative for Wolbachia.
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