Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | 11/02/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | John Overton High School |
| Observations | I found this European Earwig underground in our school grounds. The arthropod was collected approximately at 1:20pm the temperature was 50 degrees F and overcast. The ground was very mushy and wet, most of the ground was covered in leaves. Not many European Earwig were found so only one was collected. |
| Putative identification | Arthropoda Hexapoda Insecta |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | I followed the protocol from the Wolbachia database project. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | The sample did not work, the DNA extraction process failed. All controls worked, other samples worked. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Water Boatman