European Earwig

December 10, 2021

Sample information
Picture
Photos by: Hector C.
Location No map location data available.
Collection date 11/02/2021
Captive / Cultivated? Wild-caught
Group John Overton High School
Observations

I found this  European Earwig underground in our school grounds. The arthropod was collected approximately at 1:20pm the temperature was 50 degrees F and overcast. The ground was very mushy and wet, most of the ground was covered in leaves. Not many European Earwig were found so only one was collected.
Putative identification Arthropoda Hexapoda Insecta

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

I followed the protocol from the Wolbachia database project.

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

The sample did not work, the DNA extraction process failed. All controls worked, other samples worked.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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