|Location||No map location data available.|
|Captive / Cultivated?||Wild-caught|
|Group||John Overton High School|
I found this European Earwig underground in our school grounds. The arthropod was collected approximately at 1:20pm the temperature was 50 degrees F and overcast. The ground was very mushy and wet, most of the ground was covered in leaves. Not many European Earwig were found so only one was collected.
|Putative identification||Hexapoda Insecta|
|Extraction kit||DNeasy (Qiagen)|
|DNA extraction location||Abdomen|
|Single or Duplex PCR||Duplex Reaction|
|Gel electrophoresis system||Standard electrophoresis system|
|DNA stain||SYBR Safe|
I followed the protocol from the Wolbachia database project.
|Explanation of confidence level||
The sample did not work, the DNA extraction process failed. All controls worked, other samples worked.
|Wolbachia 16S sequence||
|Arthropod COI sequence||