|Captive / Cultivated?||Wild-caught|
|Group||John Overton High School|
The insect has black and a black and yellow pattern down the abdomine. Has head, thorax, and abdomine. Has brown wings.
|Putative identification||Hexapoda Insecta Hymenoptera|
|Extraction kit||DNeasy (Qiagen)|
|DNA extraction location||Abdomen|
|Single or Duplex PCR||Duplex Reaction|
|Gel electrophoresis system||Standard electrophoresis system|
|DNA stain||SYBR Safe|
Followed the protocol for The Wolbachia Project.
|Explanation of confidence level||
I am not so confidant because it has a gel smear, therefore the results could be false and the gel or DNA isolation was done incorrectly. My arthropod was in lane 3, a band came out but it had a smear. Not all of the controls worked and the experiment did not go as planned. There were a lot of smears and most of the bands did not show up. The positive control had a band for Wolbachia but not for arthropod, so something happened with the controls.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Hymenoptera was found to be negative for Wolbachia.|