Eastern Yellowjacket

December 16, 2021

Sample information
Picture
Photos by: Gracie B
Location
Collection date 11/02/2021
Captive / Cultivated? Wild-caught
Group John Overton High School
Observations

The insect has black and a black and yellow pattern down the abdomine. Has head, thorax, and abdomine. Has brown wings.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Followed the protocol for The Wolbachia Project.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I am not so confidant because it has a gel smear, therefore the results could be false and the gel or DNA isolation was done incorrectly. My arthropod was in lane 3, a band came out but it had a smear. Not all of the controls worked and the experiment did not go as planned. There were a lot of smears and most of the bands did not show up. The positive control had a band for Wolbachia but not for arthropod, so something happened with the controls.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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