Eastern Yellowjacket

Sample information
Picture	Photos by: Gracie B
Location
Collection date	11/02/2021
Captive / Cultivated?	Wild-caught
Group	John Overton High School
Observations	The insect has black and a black and yellow pattern down the abdomine. Has head, thorax, and abdomine. Has brown wings.
Putative identification	Arthropoda Hexapoda Insecta Hymenoptera
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Abdomen
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes	Followed the protocol for The Wolbachia Project.
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level	I am not so confidant because it has a gel smear, therefore the results could be false and the gel or DNA isolation was done incorrectly. My arthropod was in lane 3, a band came out but it had a smear. Not all of the controls worked and the experiment did not go as planned. There were a lot of smears and most of the bands did not show up. The positive control had a band for Wolbachia but not for arthropod, so something happened with the controls.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Hymenoptera was found to be negative for Wolbachia.

The Wolbachia Project Database