Sample information
Picture	Photos by: Fiona L.
Location
Collection date	05/02/2021
Captive / Cultivated?	Captive / Cultivated
Group	South Plainfield High School
Observations
Putative identification	Arthropoda Hexapoda Insecta Diptera Culicidae Culex
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TBE
DNA stain	Other
Gel images
Protocol notes	Used DNeasy(Qiagen) with modifications. We substituted ATL with P2 and AL with N3.
Results
Wolbachia presence	Yes
Confidence level	High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Culex was found to be postive for Wolbachia.