Paper Wasp

Sample information
Picture	Photos by: Cender Gansukh
Location
Collection date	04/10/2022
Captive / Cultivated?	Wild-caught
Group	Hampton Roads Academy
Observations	Was not moving, 4 yellow legs, large wings, visible stinger, about an inch in length, black
Putative identification	Arthropoda Hexapoda Insecta Hymenoptera
Methods
Extraction kit	Edwards Buffer
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniOne
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes	During DNA Extraction, there may have been bubbles when adding the Cell Lysis Buffer. During Gel Electrophoresis, the gel may have been poked through when adding the PCR product. The DNA pellet had a dissolved solution, and it blended to become the same color.
Results
Wolbachia presence	Yes
Confidence level	Low
Explanation of confidence level	The negative control had Wolbachia which meant there was a form of contamination.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Hymenoptera was found to be postive for Wolbachia.

The Wolbachia Project Database