Paper Wasp

Sample information

Photos by: Cender Gansukh
Collection date 04/10/2022
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy

Was not moving, 4 yellow legs, large wings, visible stinger, about an inch in length, black


Putative identification Arthropoda Hexapoda Insecta Hymenoptera


Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

During DNA Extraction, there may have been bubbles when adding the Cell Lysis Buffer. During Gel Electrophoresis, the gel may have been poked through when adding the PCR product. The DNA pellet had a dissolved solution, and it blended to become the same color.


Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

The negative control had Wolbachia which meant there was a form of contamination.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be postive for Wolbachia.
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