Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/10/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | Was not moving, 4 yellow legs, large wings, visible stinger, about an inch in length, black
|
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | During DNA Extraction, there may have been bubbles when adding the Cell Lysis Buffer. During Gel Electrophoresis, the gel may have been poked through when adding the PCR product. The DNA pellet had a dissolved solution, and it blended to become the same color. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Low |
| Explanation of confidence level | The negative control had Wolbachia which meant there was a form of contamination. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hymenoptera was found to be postive for Wolbachia. |
Water Boatman