Earwig

April 27, 2022

Sample information
Picture
Photos by: Reece D.
Location
Collection date 04/07/2022
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

The Arthropod was found under a rock, and it was moving slowly. It tried to run when the rock was removed. The bug was six legged with a long body. It had large beaded eyesIt also had long antennae like structures and pincer like structures on the back side. It had a ribbed back, no wings, and three body segments. The coloration was black, brown, and generally dark.
Putative identification Arthropoda Hexapoda Insecta Dermaptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

There were not any mistakes made in the lab that were known of. The only difficulties came from managing the materials properly, but a system was worked out that was effective.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

Due to this being the first time we have conducted this experiment, we can not be one hundred percent confident in our results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Dermaptera was found to be postive for Wolbachia.
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