Mosquito

April 27, 2022

Sample information
Picture
Photos by: Lyra S.
Location
Collection date 04/05/2022
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

It’s a mosquito. It’s not very large, and one of its wings in wrinkled. It has long, orange legs, large abdomen, rounded/small head, and it has antennas coming out of it’s head.
Putative identification Arthropoda Hexapoda Insecta Diptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Our controls didn’t turn out the way they should, so we’re not completely confident in our results. However, my result should be at least medium confident because both of mine were positive.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

Our controls were wacky.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be postive for Wolbachia.
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