Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/02/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Woodford County High School |
| Observations | Green-blue color, iridescent, six-legged, 2 antennae, stomach a deeper blue than the rest of the body. |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Fast Blast |
| Gel images |
|
| Protocol notes | Processed bug DNA was combined with the dye so that Wolbachia could be seen during gel electrophoresis, and the samples were loaded into the gel. The samples were run for ~20 minutes, then photographed and analyzed. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | By analyzing the results, we can see both the insect DNA band and the Wolbachia band as two separate bands. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Coleoptera was found to be postive for Wolbachia. |
Water Boatman