|Captive / Cultivated?||Wild-caught|
|Group||Woodford County High School|
Green-blue color, iridescent, six-legged, 2 antennae, stomach a deeper blue than the rest of the body.
|Putative identification||Hexapoda Insecta Coleoptera|
|Extraction kit||Instagene Matrix|
|DNA extraction location||Whole arthropod|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||Standard electrophoresis system|
|DNA stain||Fast Blast|
Processed bug DNA was combined with the dye so that Wolbachia could be seen during gel electrophoresis, and the samples were loaded into the gel. The samples were run for ~20 minutes, then photographed and analyzed.
|Explanation of confidence level||
By analyzing the results, we can see both the insect DNA band and the Wolbachia band as two separate bands.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Coleoptera was found to be postive for Wolbachia.|