Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 03/29/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | I found this animal near to an old tree trunk, it was slightly above the ground. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Bombyliidae |
Methods |
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| Extraction kit | Instagene Matrix |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
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| Protocol notes | Everything worked well. The extraktion was a bit difficult because I needed to figure out, where the sex organs are located. However the elektrophoresis worked well. |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | Two bands are quite clearly evident. The upper band proves that it had animal material in the DNA, the lower band proves the Wolbachia in the DNA. |
| Wolbachia 16S sequence | Download FASTA
Download AB1
GGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCCAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGNNTCNNNCCNAAAGCTA
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence | Download FASTA
Download AB1
NNTNACAGTCATCTNNTCTTGNCGTGAGNTTTNGGGNGTGCCGGGNCNNGNGAGACNGNNNCNTNNNTGCGNTCAANNANGNNNNNNGANNNNGANGCTGCNTNNAAGNNTTAANTTATATNAGNNAGNNAGNTNGGGANNNNTGNTAAGNGNTNTNTTNTNNGGGNGNTNTNGAGNNGANGNNGNAGNNGAGGGNTNNTACNANGCAAGAAAAANNAAAAAANGAANAGAAAGAANGGGGGCGCNANGGAAAGATNAGANANAGAANGAANTNNAAAAGNNGNNGGGAGGGNNNNNNNTNTNCTCTGGNNNNGGACNCNCTGCCCGGCTCGCCATGGGAATTGCTTCNNATTCANNNNANNACNCAGGAANTAACGGTGACNNAGGGNCANNANAAGANNCNNNAACCGTNAAANGACNNGAGGCTGGCGTTTATCCNTAGGCTCNGCCCCCCAGACANNCNTCACGANAANCNAAACTCAACTCANAAGNGNNGAANCCCNNCANNACTATAANNANANAATNCGNTACCNCCTGNNAACTCCCTCNNACGCNCTCCGGNNCAAACCCTGCCNCNTACCCGTATACCTGTCCNCTTACTCCCNTCATGANANTGGCNATTCCCCTNCCTCCGCAGCAGATTGCCACTNNCGNATANGNNTTCCNCNCNANNANNGCTGNGNNANCNNNNNCCCCCNCNCANNCNNNNNNCNNNGCCNNATNNNGCNNCNNNCCNTCCCNNNNN
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Bombyliidae was found to be postive for Wolbachia. |
Water Boatman