|Captive / Cultivated?||Wild-caught|
I found this animal near to an old tree trunk, it was slightly above the ground.
|Putative identification||Hexapoda Insecta Diptera Bombyliidae|
|Extraction kit||Instagene Matrix|
|DNA extraction location||Abdomen|
|Single or Duplex PCR||Duplex Reaction|
|Gel electrophoresis system||Standard electrophoresis system|
|DNA stain||SYBR Safe|
Everything worked well. The extraktion was a bit difficult because I needed to figure out, where the sex organs are located. However the elektrophoresis worked well.
|Explanation of confidence level||
Two bands are quite clearly evident. The upper band proves that it had animal material in the DNA, the lower band proves the Wolbachia in the DNA.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Bombyliidae was found to be postive for Wolbachia.|