Wolbachia_Bombyliidae_SP

Sample information

Picture
Photos by: Annina S.
Location
Collection date 03/29/2022
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

I found this animal near to an old tree trunk, it was slightly above the ground.

Putative identification Hexapoda Insecta Diptera Bombyliidae

Methods

Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Everything worked well. The extraktion was a bit difficult because I needed to figure out, where the sex organs are located. However the elektrophoresis worked well.

Results

Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Two bands are quite clearly evident. The upper band proves that it had animal material in the DNA, the lower band proves the Wolbachia in the DNA.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Bombyliidae was found to be postive for Wolbachia.
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