Wolbachia_Wil_Ilaria_Annina_22

Sample information

Picture
Photos by: Ilaria Turgut
Location
Collection date 03/29/2022
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

I found the ant under the bark of a tree. I lifted it with a pincette.

Putative identification Hexapoda Insecta Hymenoptera

Methods

Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

i made sure to crush the arthropod really well, but include the exoskeleton. the part from the arthropod that i mortared was from head to abdomen.

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level

it clearly has only one band and not two

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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