Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/29/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | I found the ant under the bark of a tree. I lifted it with a pincette. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae Lasius |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | i made sure to crush the arthropod really well, but include the exoskeleton. the part from the arthropod that i mortared was from head to abdomen. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | it clearly has only one band and not two |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Lasius was found to be negative for Wolbachia. |
Water Boatman