Wolbachia_Will22_Ilaria_Annina_Kellerrassel

Sample information

Picture
Photos by: Annina Schalch
Location
Collection date 03/29/2022
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

i found the arthropod under the bark

Putative identification Crustacea Malacostraca Isopoda Porcellionidae Porcellio Porcellio scaber

Methods

Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Probably it didn’t work because of the animal. It is difficult to locate the sex organs. Probably something went wrong during the pipette, because there is no band visible at all.

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level

there was absolutely no band

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Porcellio scaber was found to be negative for Wolbachia.
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