Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/29/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | i found the arthropod under the bark |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda Porcellionidae Porcellio Porcellio scaber |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | Probably it didn’t work because of the animal. It is difficult to locate the sex organs. Probably something went wrong during the pipette, because there is no band visible at all. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | there was absolutely no band |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Water Boatman