Brassicogethes aeneus PY1

May 10, 2022

Sample information
Picture
Photos by: Lara E.
Location
Collection date 03/29/2022
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

I found it on a dandelion flower am the edge of the forest. I collected it in the afternoon, about 2 pm.
Putative identification Arthropoda Hexapoda Insecta Coleoptera Nitidulidae Brassicogethes Brassicogethes aeneus

Methods
Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Both bands are visible
Wolbachia 16S sequence Download FASTA    Download AB1
CCCACCCTCGNCTANNNNCGNGCCNTTGGGGNCTCCCCGGGNNGGCNCAAANCNCTNNNNCGNCTTCANNNAANCANCNCCNCATANCCNNNNNTCGTCNNCNNNANNNANGTNNANNANNNNGGGNTNCGTGGAANATNGGNATNTTANNNGGTTGCTCNANAGCGNNNNGGNGNCANCNTNANCNNCAACACCAAAGCAANAAAAAACNAAACANNGCAAANAAAGCNCCGCGGNGCAANGGAANNATNANNNACAGAAACAAATCGAAAGGGGGGGNGGGTC
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
CNCCCCCNNCGAGNGNNNNCCAACTNNCNCNCNCTANGNGCTTTANGGGNNACCCCCGGCCGNAGAGCGANCCNTNTNNANTTAAANGTTCACGNAATGCAANGCACNTNNACNNTCTCCNCCNNTCGCTCTTNANNATNNANGNGNGNACNAGCTTGCGTTGNNGNNNANANCGGNNTNTCAGNNGNTTGNTCCGCNNNAGNCCNNGNCANNAACNNTTCAACCNACAACAAAAAAAAACCNCNCACCNNAAACAAGAAGCCGAGGCGCAAAGGANGCATNCANNCAAAGTCATCAAATCGAAAGCGGGGGGGGGCNGCCNTCCNCCCC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Brassicogethes aeneus was found to be postive for Wolbachia.
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