Sample information |
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Picture |
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Location | |
Collection date | 03/29/2022 |
Captive / Cultivated? | Wild-caught |
Group | KantiWil |
Observations | I found it on a dandelion flower am the edge of the forest. I collected it in the afternoon, about 2 pm. |
Putative identification | Hexapoda Insecta Coleoptera Nitidulidae Brassicogethes Brassicogethes aeneus |
Methods |
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Extraction kit | Instagene Matrix |
DNA extraction location | Abdomen |
Single or Duplex PCR | Duplex Reaction |
Gel electrophoresis system | MiniPCR |
Buffer | TAE |
DNA stain | Fast Blast |
Gel images |
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Protocol notes | |
Results |
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Wolbachia presence | Yes |
Confidence level | High |
Explanation of confidence level | |
Wolbachia 16S sequence | |
Arthropod COI sequence |
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Summary | The Brassicogethes aeneus was found to be postive for Wolbachia. |