Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/05/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | The insect was found under a tree bark. |
| Putative identification | Arthropoda Insecta Coleoptera Cerambycidae Rhagium Rhagium inquisitor |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Fast Blast |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Our confidence level is high because a Cytochromoxidase band is visible. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Rhagium inquisitor was found to be negative for Wolbachia. |
Wolbachia(-) Lasius neoniger
Wolbachia(+) Camponotus pennsylvanicus
Wolbachia(-) Aedes vexans
Wolbachia(?) Nabis americoferus
Wolbachia(-) Calliphora vicina