Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/05/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | The spider was found under a tree bark. |
| Putative identification | Arthropoda Chelicerata Arachnida Araneae Amaurobiidae Amaurobius Amaurobius fenestralis |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Fast Blast |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Our confidence level is high because a Cytochromoxidase band is visible. Gel, 4th band from the left |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Amaurobius fenestralis was found to be negative for Wolbachia. |
Joro Spider
Imported Red Fire Ant
Black carpenter ant
Flocculent tailed millipede
Tawny crazy ant