Mosquito Molly Wilson

October 24, 2022

Sample information
Picture
Photos by: Molly W.
Location
Collection date 09/20/2022
Captive / Cultivated? Wild-caught
Group South Plainfield High School
Observations

The mosquito was a female caught by the Middlesex Mosquito Commission.
Putative identification Arthropoda Hexapoda Insecta Diptera Culicidae Culex

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain Other
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There was no band at the 500 mark on the gel.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Culex was found to be negative for Wolbachia.
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