Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/06/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | College of Eastern Idaho |
| Observations | Scolops sulcipes- Partridge Bug- Crawling in dirt in the shade by the path Description: Curious, Dirt Brown with patterned markings, very camouflaged Body length: 0.9cm Found by Carly Hartzell UTM Coordinates: 12T 0492041 4797270 Arthropod: ? Wolbachia: + |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera Dictyopharidae Scolops Scolops sulcipes |
Methods |
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| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | DNA Annotated protocol in Wolbachia project Lab2: DNA Extraction Edward’s Buffer |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | We tested and retested as well as put a sample together for the Wolbachia Project Lab to identify and we received a sequence that was a match. |
| Wolbachia 16S sequence |
>Wol13 CGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTGCTATCAGGTAATGCTGAGTACTTTAAGGAAACTGCCAGTGATAAGCTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCTTTATGGAGTGGGCTACACACGTGCTACAATGGTGTCTACAATGGGCTGCAAGGTGCGCAAGCCTAAGCTAATCCCTAAAAGACATCTCAGTTCGGATTGTACTCTGCAACTCGAGTACATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGT
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence |
>CO1_13_CspecF GTTTATGATCAGGATTACTAGGAACAATAAGAAGAATAATTATTCGTATAGAACTTTTACAACCAGGATCAATAATTAAAAATGACCAAATTTATAATACCATTGTTACATCACATGCCTTCATTATAATTTTCTTTATAGTTATACCAATTATAATTGGAGGATTCGGAAATTGATTAGTTCCAATAATAATTGGTGCACCAGATATAGCATTTCCACGAATAAATAACATAAGATTTTGATTATTACCCCCCTCAATAACATTACTAATCATAAGATCAATTACAGGATCAGGAACAGGAACAGGATGAACAATTTATCCCCCCTTATCTAACCAAATAGCACATTCAGGGCCATCAGTTGACTTAACTATTTTTTCATTACATATTGCAGGAATTAGATCAATTCTAGGAGCAATTAACTTTATCTCAACTATTACAAATATACGACCAAAACCTATAACAATAGAAAATACACCACTTTTCTGTTGATCAGTTCTAATTACAGCAATTTTATTATTAGTATCATTACCAATTCTAGCAGGAGCTATTACTATACTATTAACGGATCGAAACTTTAATACATCATTTTTTGACCCATCAGGAGGAGGGGACCCAATCTTATATCAACACCTATTTTG
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Scolops sulcipes was found to be postive for Wolbachia. |
Water Boatman