Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 12/02/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Wheeler High School |
| Observations | We found this ant in a small ant hill on the grassy area of Wheeler High School. We collected the ant in the early afternoon, and the temperature was about 60 degrees Fahrenheit. There were many ants, but we only collected about 7. |
| Putative identification | Arthropoda Hexapoda Insecta |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Gel Electrophoresis Lanes- Most Right Lane= Lane 1 1- Ant that focused on in this post (Circled in Red on Gel) 2- Other ant (not covered in this post) 3- Other ant (not covered in this post) 4- Positive Arthropod Control 5- Negative Arthropod Control 6- Positive DNA Control |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | All of our controls worked as expected. We did not have any problems with my protocol, and the bands on the gel were extremely clear. Therefore, we are very confident that our arthropod is Wolbachia negative. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Insecta was found to be negative for Wolbachia. |
Water Boatman