Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 12/04/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Wheeler High School |
| Observations | I found this ant against a rock on a grassy lawn. I collected this ant at dusk, and the temperature was about 52 degrees Fahrenheit. There were many ants, but I only collected about 10. |
| Putative identification | Arthropoda Hexapoda Insecta |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | The Gel image to the left is the controls. The lane to the very left is lane 1 1- Positive DNA Control 2- Positive Arthropod Control 3- Negative Arthropod Control The Gel image to the right is the experimental data. The lane to very left is lane 1. 1- Ant that focused on in this post (Circled in Red on Gel) 2- Other ant (not covered in this post) 3- Other ant (not covered in this post) 4- Other ant (not covered in this post) |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | All of our controls worked as expected. We did not have any problems with my protocol, and the bands on the gel were extremely clear. Therefore, we are very confident that our arthropod is Wolbachia negative. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Insecta was found to be negative for Wolbachia. |
Water Boatman