NESACK-Sp23-20-MA Blattaria

April 13, 2023

Sample information
Picture
Photos by: Melanie A
Location
Collection date 01/26/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
•Environment- Under wet bed of leaves on top of soil
•Weather- Cold, sunny and wet
•Method of collection- Digging in dirt
Putative identification Arthropoda Hexapoda Insecta Blattodea

Methods
Extraction kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I have high confidence my insect has Wolbachia based off of my gel electrophoresis results. There was a clear band for both my insect and positive Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Blattodea was found to be postive for Wolbachia.
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