Hymenoptera NESACBSP23-22

Sample information

Photos by: Katherine A.
Collection date 01/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University

Time – 1:00 pm – 1:30 pm

Location – The Armstrong Post office, Art center, Science center parking lot

Environment – In tree branch

Collected by grabbing handful of pine straw, dirt, grass, and sprinkling it all over collection tray to pick out moving creatures. Breaking branches off from trees, moving bushes.

Putative identification Arthropoda Hexapoda Insecta Hymenoptera


Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.


Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I placed my confidence level between the middle because I’m unsure my results could be trusted due to my Gel electrophoresis results. I would say that my controls worked.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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