Hymenoptera NESACBSP23-22

September 7, 2023

Sample information
Picture
Photos by: Katherine A.
Location
Collection date 01/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Time – 1:00 pm – 1:30 pm

Location – The Armstrong Post office, Art center, Science center parking lot

Environment – In tree branch

Collected by grabbing handful of pine straw, dirt, grass, and sprinkling it all over collection tray to pick out moving creatures. Breaking branches off from trees, moving bushes.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I placed my confidence level between the middle because I’m unsure my results could be trusted due to my Gel electrophoresis results. I would say that my controls worked.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
Report Inappropriate Post