Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/23/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Time – 1:00 pm – 1:30 pm Location – The Armstrong Post office, Art center, Science center parking lot Environment – In tree branch Collected by grabbing handful of pine straw, dirt, grass, and sprinkling it all over collection tray to pick out moving creatures. Breaking branches off from trees, moving bushes. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | I placed my confidence level between the middle because I’m unsure my results could be trusted due to my Gel electrophoresis results. I would say that my controls worked. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |
Sehirus cinctus
Melanoplus bruneri
Pterostichus permundus
Melanoplus Spretus
Centipede- Lithobius forficatus