GRE-ACF-Sp23-#10 Blattaria

April 19, 2023

Sample information
Picture
Photos by: Kalia C.
Location
Collection date 01/25/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
The weather was clear skies with a degree of 60 Celsius
The blattaria was collect in grass and bushes. My group and I used the bowl to scoop up dirt from under branches and looked through the dirt for insect. We also collected insects with the Aerial Insect Net scooping up grass and flowers. I then transferred the insects into my kill jar.
Putative identification Arthropoda Hexapoda Insecta Blattodea

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I believe that my insect is not Wolbachia positive because the controls came out how they were supposed to. Meaning there was no cross-contamination in my sample.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Blattodea was found to be negative for Wolbachia.
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