Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 04/05/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Arroyo High School |
| Observations | We found the pincher roaming around and used tweezers and a cup to capture it. In order to preserve the the spider we place it into alcohol. It was wandering in a shady area, it was dark brown in color and was very still as we approached it. |
| Putative identification | Arthropoda Hexapoda Insecta Dermaptera Forficulidae Forficula Forficula auricularia |
Methods |
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| Extraction kit | none |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | We ran a pcr, using the body of the insect to collect as much DNA as possible. We had a negative control and a postive control. Lane 3 is where we tested the pincher for wolbachia but unfortunately something happened and none of the wells showed up. Even if any fragments did show up, out tube’s labels were erased so out data is inconclusive. |
Results |
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| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | Since out gel did not come out with any results and if it did it was faint lines we are not confident. Out controls didn’t come out the way they were supposed to as well which meant that we did something wrong along the process. Possibly not collecting enough DNA or missing a step in out protcol. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
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| Summary | |
Water Boatman