forficula auricularia (pincher bug) – 51845

May 25, 2023

Sample information
Picture
Photos by: Jenny T.
Location
Collection date 04/05/2023
Captive / Cultivated? Wild-caught
Group Arroyo High School
Observations

We found the pincher roaming around and used tweezers and a cup to capture it. In order to preserve the the spider we place it into alcohol. It was wandering in a shady area, it was dark brown in color and was very still as we approached it.
Putative identification Arthropoda Hexapoda Insecta Dermaptera Forficulidae Forficula Forficula auricularia

Methods
Extraction kit none
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

We ran a pcr, using the body of the insect to collect as much DNA as possible. We had a negative control and a postive control. Lane 3 is where we tested the pincher for wolbachia but unfortunately something happened and none of the wells showed up. Even if any fragments did show up, out tube’s labels were erased so out data is inconclusive.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

Since out gel did not come out with any results and if it did it was faint lines we are not confident. Out controls didn’t come out the way they were supposed to as well which meant that we did something wrong along the process. Possibly not collecting enough DNA or missing a step in out protcol.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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