Prenolepis imparis

May 26, 2023

Sample information
Picture
Photos by: Ron N
Location
Collection date 04/07/2023
Captive / Cultivated? Wild-caught
Group Arroyo High School
Observations

Prenolepis imparis 4mm length

Metasoma ~1.8-1.9 mm

Head ~.8-.9mm

4 visible legs (some legs possibly lost during transportation)

small mesosoma ~1 mm

2 long antennas

Dark brown color

3 white stripes on the metasoma

 

 

 
Putative identification Arthropoda Hexapoda Insecta

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Our gel results showed no bands for Wolbachia. When compared to other groups who had similar insects to us, they also yielded the same results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Insecta was found to be negative for Wolbachia.
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