Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/07/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Arroyo High School |
| Observations | Prenolepis imparis 4mm length Metasoma ~1.8-1.9 mm Head ~.8-.9mm 4 visible legs (some legs possibly lost during transportation) small mesosoma ~1 mm 2 long antennas Dark brown color 3 white stripes on the metasoma
|
| Putative identification | Arthropoda Hexapoda Insecta |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Our gel results showed no bands for Wolbachia. When compared to other groups who had similar insects to us, they also yielded the same results. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Insecta was found to be negative for Wolbachia. |
Melanoplus Spretus
Centipede- Lithobius forficatus
Fly- Pollen pediculata
Cricket- Acheta domesticus
Ant- Tetramorium immigrant