Chilopoda; Centipede by group 4

May 15, 2023

Sample information
Picture
Photos by: Jolly J., Alexia G.
Location
Collection date 04/06/2023
Captive / Cultivated? Wild-caught
Group Arroyo High School
Observations
  • Found under and damp block of wood.
  • Surrounded by damp wood chips
Putative identification Arthropoda Myriapoda Chilopoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level
Explanation of confidence level

We had made a lot of human errors, even by comparison to the groups who’ve had similar insects. One of our group members had stabbed through the separating wall between the wells (wells 4 & 5). This caused the DNA samples to mix, but luckily the wells only contained our control samples. Since it was only our control sample that had been botched we were able to go to another group and use their data. But we are still unsure if the damage caused to 4 and 5 had affected any other wells.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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