Lady bug JJJ2

May 15, 2023

Sample information
Picture
Photos by: Henry X.
Location
Collection date 04/06/2023
Captive / Cultivated? Wild-caught
Group Arroyo High School
Observations

Dark red orange elytra with symmetrical black spots, black striped abdomen, six legs intact, wings intact
Putative identification Arthropoda Hexapoda Insecta Coleoptera Coccinellidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

We made a 1% agarose gel for this protocol, with a positive arthropod control, negative arthropod control, and a positive DNA control. We also tested our samples for Arthropod CO1 to verify that we actually got arthropod DNA.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

Our confidence in our results is low because there were no bands for our JJJ1(our other sample) for our Arthropod CO1 gel, so we may have improperly done our DNA extraction or PCR protocols. In addition, our DNA control didn’t have a band for the Wolbachia gel despite appearing on the Arthropod gel.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coccinellidae was found to be negative for Wolbachia.
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