Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/24/2020 |
| Captive / Cultivated? | Wild-caught |
| Group | Hillsboro High School |
| Observations | I collected this ant from under old damp wood. There were many more ants in this environment along with many other insect species. This ant was collected at around 5:30 pm and it was in the lower 70s outside. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | I am very confident that this collared ant does not have Wolbachia because when looking at the gel electrophoresis results, there was no band present for Wolbachia positive. The results were viewed under different contrast levels, and under each level, there was no band present, so it is clear that there was no Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Formicidae was found to be negative for Wolbachia. |
Water Boatman