Sample Information: Spider arthropod

May 27, 2023

Sample information
Picture
Photos by: Marakii W.
Location
Collection date 04/13/2023
Captive / Cultivated? Wild-caught
Group Arroyo High School
Observations
Putative identification Arthropoda Chelicerata

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location body without wings
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

I am not confident because we aren’t Wolbachia positive and if they did have Wolbachia it would have shown since from our gel we can see that the DNA ladder did in fact work as intended.
Wolbachia 16S sequence
Arthropod COI sequence
The Chelicerata was found to be negative for Wolbachia.
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Chelicerata was found to be negative for Wolbachia.
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