Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/13/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Arroyo High School |
| Observations | |
| Putative identification | Arthropoda Chelicerata |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | body without wings |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | I am not confident because we aren’t Wolbachia positive and if they did have Wolbachia it would have shown since from our gel we can see that the DNA ladder did in fact work as intended. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
The Chelicerata was found to be negative for Wolbachia.
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Chelicerata was found to be negative for Wolbachia. |
Water Boatman