Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/07/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Thrive Scholars |
| Observations | small, thin, red caterpillar found in the dirt of a garden |
| Putative identification | Arthropoda Hexapoda Insecta Lepidoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | We followed the protocol on the website. Top gel: arthropod DNA primer Bottom gel: wolbachia DNA primer sample in the second lane for both gels |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | There was clearly no wolbachia DNA fragments present, but the top half of the gel with the arthropod primer makes us doubt the validity of the entire experiment since it was also unclear if there was arthropod DNA in the sample. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Lepidoptera was found to be postive for Wolbachia. |
Water Boatman